normal skin fibroblast cell line  (ATCC)

Journal: EMBO Reports

Article Title: ATAD2 drives melanoma growth and progression and inhibits ferroptosis

doi: 10.1038/s44319-025-00660-w

Figure Lengend Snippet: ( A ) The Gene Expression Profiling Interactive Analysis (GEPIA) data was used for plotting mRNA expression of family IV of bromodomain-containing members. mRNA expression of BRPF1, BRPF2, BRPF3, BRD7, BRD9, and ATAD2b is shown in melanoma patient samples ( n = 461) and normal skin samples ( n = 558). ( B , C ) Human Protein Atlas showing ATAD2 protein expression intensity in different patient samples. ( D ) List of transcription factors with predicted DNA binding sites on the ATAD1 promoter DNA sequence (1 kb upstream from the transcription start site) generated using PROMO search. ( E ) TCGA melanoma data showing the correlation between ATAD2 and E2F1 mRNA expression. ( F ) In the indicated melanoma cell lines ATAD2 mRNA expression was measured using RT-qPCR and plotted relative to primary human fibroblast cells. ACTINB was used as a normalization control (left) and ATAD2 protein expression in the shown cells was measured via western blotting. ACTINB was used as loading control (right). ( G ) The indicated cell lines were treated with 5μM of BAY-850 for 3 days, and viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Relative cell viability in treated condition relative to DMSO-treated condition is presented, ns P = 0.2244, ** P = 0.0013, **** P = < 0.0001, **** P = < 0.0001, from left to right. ( A ) P value was calculated using ANOVA (Analysis of Variance) for differential expression analysis between tumor and normal tissues. ( G ) P value was calculated using unpaired Student’s t test using three independent replicates.

Article Snippet: Melanoma cell lines (A375, M14, SKMEL-2, SKMEL-103), HEK-293T and primary human fibroblast cells were purchased from American Type Culture Collection (ATCC), Sigma and Cytion as listed in Reagents and Tools Table and maintained in a humidified atmosphere of 5% CO 2 at 37 °C in Dulbecco’s modified Eagle medium (DMEM; Life Technologies, Carlsbad, CA, USA) or Roswell Park Memorial Institute-1640 Medium (RPMI)-1640 Medium; (Life Technologies), each supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (both from Life Technologies).

Techniques: Gene Expression, Expressing, Binding Assay, Sequencing, Generated, Quantitative RT-PCR, Control, Western Blot, MTT Assay, Quantitative Proteomics

Journal: Biomolecules & Therapeutics

Article Title: Liposomal Hyaluronic Acid Enhances Skin Permeation and Hydration: Evidence from In Vitro, Ex Vivo, and In Vivo Studies

doi: 10.4062/biomolther.2025.163

Figure Lengend Snippet: Effects of LPS-HA on extracellular matrix proteins (Type I/III collagens and elastin) in human fibroblasts. (A) Type I collagen, (B) Type III collagen, and (C) Elastin expression in human normal fibroblasts treated with liposomal hyaluronic acid (LPS-HA; 0.1-0.5%) or hyaluronic acid (HA; 0.1-0.5%). Vitamin C (100 μg/mL) served as the positive control. Data are expressed as mean ± SEM (n=3). Bars indicate expression relative to untreated control (set at 100%). * p <0.05 ** p <0.01 *** p <0.001 compared with control.

Article Snippet: Extracellular matrix protein expression analysis (real-time PCR): Human normal fibroblasts (ATCC) were cultured into 60-mm dishes (Nunc) and incubated at 37°C in a 5% CO 2 incubator for 24 h. The medium was replaced with serum-free IMDM (Welgene), and test samples were applied at the indicated concentrations for another 24 h. Cells were washed with PBS and treated with QIAzolTM Lysis Reagent (QIAGEN) to extract RNA.

Techniques: Expressing, Positive Control, Control